GSM1277197: RNASeq_riboM_contDevB_N2_34hr; Caenorhabditis elegans; RN... - SRA

SRX385630: GSM1277197: RNASeq_riboM_contDevB_N2_34hr; Caenorhabditis elegans; RNA-Seq
2 ILLUMINA (Illumina HiSeq 2000) runs: 55.3M spots, 2.8G bases, 1.8Gb downloads

Submitted by: Gene Expression Omnibus (GEO)

Study: Developmental Timecourses total-RNA sequencing [Ribosome repleted total RNA]

PRJNA230377 • SRP033412 • All experiments • All runs

show Abstracthide Abstract

We report the presence of extensive, transcriptionally controlled oscillations in the C. elegans, developmental transcriptome. Furthermore, using ribosome profiling, we show that these oscillating transcripts are actively translated. Overall design: Examination of two timecourses that were collected over C. elegans development and analyzed by RNA-seq of "RiboMinus" libraries

Sample: RNASeq_riboM_contDevB_N2_34hr

SAMN02428708 • SRS509736 • All experiments • All runs

Organism: Caenorhabditis elegans

Library:

Instrument: Illumina HiSeq 2000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: SINGLE

Construction protocol: total RNA isolation was performed using Tri-Reagent (MRC) Library preparation was performed using the ScriptSeq v2 RNA-Seq library preparation kit (Epicentre) according to the manufacturer's protocol

Experiment attributes:

GEO Accession: GSM1277197

Links:

External link: GEO Sample GSM1277197

NCBI link: NCBI Entrez (gds)

Runs: 2 runs, 55.3M spots, 2.8G bases, 1.8Gb

Run	# of Spots	# of Bases	Size	Published
SRR1041255	50,000,000	2.6G	1.6Gb	2014-03-03
SRR1041256	5,315,601	271.1M	183.1Mb	2014-03-03

ID:: 553482

SRA

Sequence Read Archive

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